Uber shot back against the removal of its license, saying that London is closed to innovation and that it will appeal the decision and challenge it in the courts.
In an e-mail to Uber employees, Mr Khosrowshahi said he thought the London decision was unfair, but "the truth is that there is a high cost to a bad reputation".
City authorities accused the company of failing to ensure "passenger safety" after a string of allegations the company failed to report sex attacks by drivers. "I think the London cabbies have probably been a little slow in adjusting to the new system", Ross said.
The decision is a victory for the city's traditional black cab industry, which has been hurt by the proliferation of Uber drivers and has pushed for tighter regulation of the San Francisco-based ride-hailing service.
The mayor acknowledged the popularity of Uber in the capital but said "it would be wrong for TfL to license Uber if there was any way this could pose a threat to Londoners' safety or security".
Considering the fact that the company will no longer be able to offer the service in London from October 1, Uber users in London have made their voices heard by signing an #Online Petition created by the #American Company.
In the petition, Uber had pointed out that its service is used by 3.5 million people in the city and in addition to that, 40,000 drivers make a living through the company.
In a recent decision, Transport for London singled out Uber's approach to reporting serious criminal offenses and how it conducts background checks on drivers.
Responding to the petition, Fred Jones, Uber's United Kingdom head of cities, told the BBC: "I think people realise that this decision by the mayor and Transport for London is actually because they have caved to pressure from a small number of individuals and groups that want to protect the status quo and reduce consumer choice and competition from London".
The company has already been forced to leave several countries, including Denmark and Hungary, and has faced regulatory battles in multiple U.S. states and countries around the world.
Bradford council is "continuing to monitor" controversial online private hire firm Uber after the company lost its licence to operate in London.
Uber operates in more than 600 locations around the world, including more than 40 towns and cities in the UK. Uber has been criticised by unions and lawmakers too and been embroiled in legal battles over workers' rights.